7ABV

Structure of the S1-cleaved mouse Notch1 Negative Regulatory Region (NRR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.06998
Spacegroup name	P 42 21 2
Unit cell lengths	65.724, 65.724, 161.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.885 - 2.065
Rwork	0.196
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eto
RMSD bond length	0.006
RMSD bond angle	1.501
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.886	2.296
High resolution limit [Å]	2.065	2.065
Rmerge	0.105	1.431
Rmeas	0.109	1.496
Rpim	0.030	0.430
Number of reflections	15144	757
<I/σ(I)>	14.6
Completeness [%]	91.5	64.6
Redundancy	12.9	11.8
CC(1/2)	0.999	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.0 M sodium chloride, 0.1 M sodium acetate pH 4.6