7AB8

Crystal structure of a GDNF-GFRalpha1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	125.067, 55.544, 70.961
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.760 - 2.200
R-factor	0.2006
Rwork	0.199
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fub
RMSD bond length	0.006
RMSD bond angle	0.747
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.780	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.056	0.371
Rmeas	0.079	0.525
Rpim	0.056	0.371
Number of reflections	25823	1368
<I/σ(I)>	12.3	3.15
Completeness [%]	91.9	53.94
Redundancy	2	2
CC(1/2)	0.997	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	295	Tris, PEG 20K, MeCN, NaCl