7A9X

Structure of yeast Rmd9p in complex with 16nt target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	106.250, 106.250, 128.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.010 - 2.450
R-factor	0.2004
Rwork	0.199
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a9w
RMSD bond length	0.002
RMSD bond angle	0.429
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.020	2.500
High resolution limit [Å]	2.450	2.450
Rmerge	0.077	2.809
Number of reflections	59634	4420
<I/σ(I)>	18.11
Completeness [%]	100.0	100
Redundancy	10.7	10.25
CC(1/2)	1.000	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293.15	Na-Cacodylate, NaCl, LiSO4, CaAcetate, MgCl2, PEG8000