7A68
proteinase K crystallized from 0.5 M NaNO3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.320, 68.320, 108.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.900 - 2.550 |
| R-factor | 0.1496 |
| Rwork | 0.147 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2id8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.827 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.117 | 0.350 |
| Rmeas | 0.130 | 0.387 |
| Number of reflections | 8092 | 1199 |
| <I/σ(I)> | 5.8749 | 2 |
| Completeness [%] | 92.0 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein concentration: 10mg/ml. 0,2 M Tris pH 8, 0.5 M NaNO3 |
