7A5T
Crystal structure of A55E mutant of BlaC from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.990, 54.356, 53.687 |
Unit cell angles | 90.00, 91.94, 90.00 |
Refinement procedure
Resolution | 38.210 - 1.400 |
R-factor | 0.14896 |
Rwork | 0.148 |
R-free | 0.17725 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gdn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.781 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.210 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rpim | 0.078 | 0.344 |
Number of reflections | 42718 | 2050 |
<I/σ(I)> | 5.2 | |
Completeness [%] | 96.9 | 94.2 |
Redundancy | 2.5 | 2.3 |
CC(1/2) | 0.985 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8 M Disodium Succinate |