7A0S

50S Deinococcus radiodurans ribosome bounded with mycinamicin I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	I 2 2 2
Unit cell lengths	169.995, 410.736, 697.778
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.670 - 3.220
R-factor	0.2252
Rwork	0.224
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pio
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.270
High resolution limit [Å]	3.210	8.700	3.210
Rmerge	0.182	0.064	1.574
Rmeas	0.207	0.075	1.784
Rpim	0.096	0.038	0.823
Total number of observations	1739141
Number of reflections	389019	19899	19343
<I/σ(I)>	4.7
Completeness [%]	99.6	98.1	99.6
Redundancy	4.5	3.8	4.5
CC(1/2)		0.995	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ribosome solution containing 0.0065 mM (180 A/ml) of D50S in 10 mM Hepes pH=7.8, 15 mM MgCl2 and 75 mM NH4Cl crystallization buffer was mixed with 10 mM spermidine, 1 % ethanol and 0.5 % 2-ethyl-1,3-hexanediol precipitants. A 0.005 ml crystallization drop was hanged over 10 % ethanol and 5% 2-ethyl-1,3-hexanediol in ddw reservoir.