Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.959, 66.479, 250.843 |
| Unit cell angles | 90.00, 100.10, 90.00 |
Refinement procedure
| Resolution | 46.730 - 2.650 |
| Rwork | 0.212 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jnk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.771 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.115 | 0.529 |
| Number of reflections | 81570 | 4427 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.2M Na malonate, 0.1M Bis-Tris propane pH6.5, 20% PEG3,350 |
