6ZYJ
Crystal structure of Hsc70 ATPase domain in complex with ADP and calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.677, 78.039, 75.377 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 73.710 - 1.850 |
| R-factor | 0.2192 |
| Rwork | 0.218 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iuc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.172 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.940 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.120 | 1.005 |
| Number of reflections | 71566 | 4400 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.633 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 24% PEG3350, 0.2M Ca acetate |
