6ZXS
Cold grown Pea Photosystem I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-08-24 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 190.197, 201.786, 213.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.630 - 3.000 |
R-factor | 0.24 |
Rwork | 0.239 |
R-free | 0.26970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l8r |
RMSD bond length | 0.005 |
RMSD bond angle | 1.754 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.326 | |
Number of reflections | 162077 | 7998 |
<I/σ(I)> | 5.8 | |
Completeness [%] | 98.8 | |
Redundancy | 7 | |
CC(1/2) | 0.997 | 0.066 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | di-potassium phosphate, Tris, PEG400, glycerol, L-glutathione, octyl glucose neopentyl glycol |