6ZWB
Z-SBTub3 photoswitch bound to tubulin-DARPin D1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.869, 91.724, 83.006 |
| Unit cell angles | 90.00, 97.06, 90.00 |
Refinement procedure
| Resolution | 45.039 - 1.747 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nm5 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.039 | 1.973 |
| High resolution limit [Å] | 1.747 | 1.747 |
| Rmerge | 0.105 | 0.764 |
| Rmeas | 0.119 | 0.877 |
| Rpim | 0.054 | 0.424 |
| Number of reflections | 68165 | 3408 |
| <I/σ(I)> | 9.2 | 1.8 |
| Completeness [%] | 61.3 | 10 |
| Redundancy | 4.5 | 4.1 |
| CC(1/2) | 0.997 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3000, ammonium sulfate, bis-tris methane |
