6ZWA
CLIP peptide bound to chicken MHC class II molecule (BL-2) from B19 haplotype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-01-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.863, 60.536, 53.855 |
| Unit cell angles | 90.00, 107.88, 90.00 |
Refinement procedure
| Resolution | 55.800 - 1.680 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t3y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.900 | 55.840 | 1.770 |
| High resolution limit [Å] | 1.680 | 5.300 | 1.680 |
| Rmerge | 0.097 | 0.062 | 1.972 |
| Rmeas | 0.106 | 0.068 | 2.138 |
| Rpim | 0.042 | 0.028 | 0.817 |
| Total number of observations | 9879 | 51686 | |
| Number of reflections | 50834 | 1731 | 7737 |
| <I/σ(I)> | 9.2 | 22.3 | 1.1 |
| Completeness [%] | 95.0 | 98.7 | 99.7 |
| Redundancy | 6.5 | 5.7 | 6.7 |
| CC(1/2) | 0.997 | 0.995 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M TRIS pH 8.5, 20.0% w/v PEG 4000, 200mM CaCl2 |
