6ZV6
Human RIO1(kd)-StHA late pre-40S particle, structural state B (post 18S rRNA cleavage)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.9 |
| Number of Particle | 2126610 |
| Software | RELION,REFMAC,PHENIX,SerialEM,Gctf,Coot,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
