6ZTL
E. coli 70S-RNAP expressome complex in collided state bound to NusG
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Number of Particle | 684211 |
| Software | RELION,EPU,CTFFIND,UCSF Chimera,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
