6ZTB

Crystal Structure of human P-Cadherin EC1_EC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	C 1 2 1
Unit cell lengths	120.889, 76.522, 46.208
Unit cell angles	90.00, 107.79, 90.00

Refinement procedure

Resolution	57.550 - 1.400
R-factor	0.1989
Rwork	0.198
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l3w
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.550	57.550	1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.022	0.018	0.523
Rmeas	0.026	0.021	0.620
Number of reflections	78188	915	5758
<I/σ(I)>	23.72	67.59	2.06
Completeness [%]	99.2	98.4	99.6
Redundancy	3.328	3.502	3.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	3.655M NaCl, 85mM HEPES, 15.0% GLYCEROL