6ZSJ

The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.919550
Spacegroup name	C 1 2 1
Unit cell lengths	117.340, 35.660, 168.560
Unit cell angles	90.00, 94.50, 90.00

Refinement procedure

Resolution	42.140 - 2.000
R-factor	0.2497
Rwork	0.247
R-free	0.29080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5szi
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.140	42.140	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.067	0.027	1.448
Rmeas	0.073	0.030	1.575
Total number of observations	314837
Number of reflections	47563	587	3514
<I/σ(I)>	12.3	39.42	1.12
Completeness [%]	99.2	97.5	100
Redundancy	6.619	5.928	6.539
CC(1/2)	0.999	0.999	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M Mes pH 6.5 10 % (w/v) Peg-MME5000 12% (v/v) 1-propanol