6ZSI

The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.919532
Spacegroup name	C 1 2 1
Unit cell lengths	116.360, 35.380, 165.670
Unit cell angles	90.00, 93.76, 90.00

Refinement procedure

Resolution	46.108 - 1.914
R-factor	0.2252
Rwork	0.223
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5szi
RMSD bond length	0.009
RMSD bond angle	1.231
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.108	46.108	1.960
High resolution limit [Å]	1.910	8.560	1.910
Rmerge	0.068	0.032	0.872
Rmeas	0.071	0.034	0.948
Total number of observations	591373
Number of reflections	52601	659	3671
<I/σ(I)>	19.75	61.69	2.07
Completeness [%]	99.5	99.1	93.3
Redundancy	11.243	12.382	6.47
CC(1/2)	0.999	0.999	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M Mes pH 6.0 5% w/v PEG 3000 30% v/v PEG 200