6ZQO
EYFP mutant - F165G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.888, 57.888, 168.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.200 |
| R-factor | 0.2585 |
| Rwork | 0.254 |
| R-free | 0.33960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yfp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.542 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.730 | 2.200 |
| Rmerge | 0.060 | 0.040 | 0.794 |
| Rmeas | 0.066 | 0.044 | 0.876 |
| Rpim | 0.028 | 0.019 | 0.362 |
| Total number of observations | 83201 | ||
| Number of reflections | 14573 | 1475 | 1457 |
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 94.7 | 85.6 | 98.4 |
| Redundancy | 5.7 | 5.1 | 5.5 |
| CC(1/2) | 0.998 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5.7 mg/ml in 20mM Tris 8.0 200 mM NaCl mixed with a 1.44M (NH4)2SO4, 60mM Bicine pH 9.0 reservoir solution. |
