6ZMU

Crystal structure of the germline-specific thioredoxin protein Deadhead (Thioredoxin-1) from Drospohila melanogaster, P43212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	111.827, 111.827, 106.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.430 - 1.950
R-factor	0.187
Rwork	0.185
R-free	0.22035
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xwa
RMSD bond length	0.017
RMSD bond angle	1.753
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.430	2.054
High resolution limit [Å]	1.950	1.970
Number of reflections	43714	2187
<I/σ(I)>	18.1	1.5
Completeness [%]	96.7
Redundancy	10.8
CC(1/2)	1.000	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium citrate, pH 5.0, 3.2 M ammonium sulfate