6ZL3
CRYSTAL STRUCTURE OF HRAS IN COMPLEX WITH COMPOUND 18 and GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-04 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 93.067, 93.067, 55.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.530 - 2.031 |
R-factor | 0.2026 |
Rwork | 0.202 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l8z |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.500 | 2.165 |
High resolution limit [Å] | 2.000 | 2.013 |
Number of reflections | 14959 | 748 |
<I/σ(I)> | 17 | |
Completeness [%] | 89.8 | |
Redundancy | 9.4 | |
CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 278 | 160mM Calcium acetate 20% Glycerol 14.4% PEG8000 80mM Sodium cacodylate |