6ZK2
Plant nucleoside hydrolase - ZmNRh2b in complex with forodesine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.920, 148.980, 88.510 |
| Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
| Resolution | 30.120 - 2.200 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kpo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.163 | 0.520 |
| Number of reflections | 59871 | 5446 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.987 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | PEG 4000, Tris |
