6ZK2
Plant nucleoside hydrolase - ZmNRh2b in complex with forodesine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.920, 148.980, 88.510 |
Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
Resolution | 30.120 - 2.200 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kpo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.800 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.163 | 0.520 |
Number of reflections | 59871 | 5446 |
<I/σ(I)> | 6.4 | |
Completeness [%] | 97.6 | |
Redundancy | 3.6 | |
CC(1/2) | 0.987 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | PEG 4000, Tris |