6ZIP
Crystal Structure of Two-Domain Laccase mutant R240A from Streptomyces griseoflavus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.861000 |
Spacegroup name | P 1 |
Unit cell lengths | 76.873, 94.838, 116.106 |
Unit cell angles | 90.21, 90.24, 91.46 |
Refinement procedure
Resolution | 47.400 - 2.050 |
R-factor | 0.1891 |
Rwork | 0.188 |
R-free | 0.20810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lhl |
RMSD bond length | 0.009 |
RMSD bond angle | 1.008 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.085 | 0.720 |
Number of reflections | 200840 | 14801 |
<I/σ(I)> | 7.09 | 1.28 |
Completeness [%] | 97.6 | 97.2 |
Redundancy | 2.67 | |
CC(1/2) | 0.990 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.3 | 285 | 20 % v/v PEG Smear High 0.1 M Bicine, pH 9.3 |