6ZHU

Yeast Uba1 in complex with Ubc3 and ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.873
Spacegroup name	P 1 21 1
Unit cell lengths	75.636, 152.408, 252.567
Unit cell angles	90.00, 90.45, 90.00

Refinement procedure

Resolution	49.810 - 3.180
R-factor	0.2192
Rwork	0.217
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmm
RMSD bond length	0.016
RMSD bond angle	1.450
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.810	3.294
High resolution limit [Å]	3.180	3.180
Number of reflections	96004	9585
<I/σ(I)>	7.1
Completeness [%]	99.7
Redundancy	3.8
CC(1/2)	0.994	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium chloride, PEG 3350