6ZG4
Structure of M1-StaR-T4L in complex with HTL0009936 at 2.35A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.03319 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.470, 65.450, 154.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.980 - 2.330 |
R-factor | 0.205 |
Rwork | 0.204 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y00 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.982 | 2.484 |
High resolution limit [Å] | 2.353 | 2.353 |
Rpim | 0.194 | 1.525 |
Number of reflections | 20575 | 674 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 89.2 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1M NaHEPES pH 7.4-7.8, 0.1M di-Ammonium hydrogenphosphate, 30-38% PEG300 |