6ZFL

High resolution structure of VEGF-A 12:107 crystallized in tetragonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.272
Spacegroup name	P 41
Unit cell lengths	88.340, 88.340, 40.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.170 - 1.600
R-factor	0.1652
Rwork	0.164
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1flt
RMSD bond length	0.009
RMSD bond angle	1.023
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX (1.18_3855)
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.170	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.044	0.693
Rmeas	0.092	0.838
Rpim	0.049	0.464
Number of reflections	41413	6017
<I/σ(I)>	7.9	1.6
Completeness [%]	99.6	99.7
Redundancy	3.3	3.1
CC(1/2)	0.996	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	(VEGF)2:2 PEPTIDE_2C COMPLEX PURIFIED ON SEC AND CONCENTRATED AT 17MG/ML IN TRIS/HCL 10MM PH 8.5. (SEE ID 6Z3F) mix 1UL OF COMPLEX WITH 1 UL OF RESERVOIR : TRIS/HCL 100MM PH 8.5 / MPD 42% (V/V) / (NH4)2PO4 200MM