6ZER

Crystal structure of receptor binding domain of SARS-CoV-2 Spike glycoprotein in complex with EY6A Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	P 31 2 1
Unit cell lengths	166.404, 166.404, 270.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.220 - 3.800
R-factor	0.2139
Rwork	0.212
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yla
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	144.000	3.870
High resolution limit [Å]	3.800	3.800
Rmerge	0.227
Number of reflections	43446	2141
<I/σ(I)>	7.3	0.4
Completeness [%]	100.0
Redundancy	19.8
CC(1/2)	0.998	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.02 M NaH2PO4, 0.98 M K2HPO4