6ZEF

Structure of PP1(7-300) bound to Phactr1 (516-580) at pH 5.25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	47.510, 57.550, 89.614
Unit cell angles	78.08, 74.67, 81.66

Refinement procedure

Resolution	56.050 - 1.940
R-factor	0.1829
Rwork	0.181
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.005
RMSD bond angle	0.841
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.050	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.133	0.579
Rmeas	0.141	0.640
Rpim	0.047	0.260
Number of reflections	65136	6061
<I/σ(I)>	9.77	2.73
Completeness [%]	98.5	91.97
Redundancy	8
CC(1/2)	0.880	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.25	293	1M LiCl, 0.1 M tri-sodium citrate pH 5.25 and 10 % PEG 6000