6ZC3
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-05-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 61 |
Unit cell lengths | 87.276, 87.276, 57.844 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.840 - 1.670 |
R-factor | 0.1686 |
Rwork | 0.167 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f0a |
RMSD bond length | 0.002 |
RMSD bond angle | 0.978 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.840 | 1.710 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmeas | 0.005 | 0.077 |
Number of reflections | 29202 | 2161 |
<I/σ(I)> | 19.1 | |
Completeness [%] | 99.9 | |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2 M ammonium sulphate, 0.1 M citric acid pH 5.0 |