6ZBU
Crystal structure of an NCoR1BBD2-BCL6BTB chimera in complex with the NcoR1 BBD1 corepressor peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 198.575, 198.575, 113.067 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.810 - 2.460 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.25930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xyx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.320 | 2.520 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.150 | |
| Number of reflections | 82349 | 6006 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 5.9 |
| CC(1/2) | 0.997 | 0.345 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.34M Ammonium sulfate 0.67%(v/v) MPD 0.1M HEPES/ Sodium hydroxide pH 7.5 |
