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6ZAY

Crystal structure of Atg16L in complex with GDP-bound Rab33B

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X10SA
Synchrotron siteSLS
BeamlineX10SA
Temperature [K]100
Detector technologyPIXEL
Collection date2013-06-29
DetectorDECTRIS PILATUS 6M-F
Wavelength(s)0.97714
Spacegroup nameP 21 21 21
Unit cell lengths57.010, 132.110, 154.370
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.572 - 2.400
R-factor0.1865
Rwork0.185
R-free0.21140
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1Z06 and 3Q8T
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwareREFMAC (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.5722.486
High resolution limit [Å]2.4002.400
Rmerge0.117
Number of reflections464524577
<I/σ(I)>16.22
Completeness [%]100.099.89
Redundancy13.113.7
CC(1/2)0.971
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION6.2277.1522% PEG3350, 0.2M potassium thiocyanate and 0.1M sodium potassium phosphate

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PDB entries from 2024-11-13

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