6ZAY
Crystal structure of Atg16L in complex with GDP-bound Rab33B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97714 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.010, 132.110, 154.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.572 - 2.400 |
R-factor | 0.1865 |
Rwork | 0.185 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1Z06 and 3Q8T |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.572 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.117 | |
Number of reflections | 46452 | 4577 |
<I/σ(I)> | 16.22 | |
Completeness [%] | 100.0 | 99.89 |
Redundancy | 13.1 | 13.7 |
CC(1/2) | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 277.15 | 22% PEG3350, 0.2M potassium thiocyanate and 0.1M sodium potassium phosphate |