6Z8C

Crystal Structure of the Voltage-Gated Sodium Channel NavMs (F208L) in complex with N-desmethyltamoxifen (3.2 A resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96864
Spacegroup name	I 4 2 2
Unit cell lengths	109.221, 109.221, 211.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.510 - 3.200
R-factor	0.2541
Rwork	0.254
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvx
RMSD bond length	0.008
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (19-MAR-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.510	3.420
High resolution limit [Å]	3.200	3.200
Number of reflections	10937	1934
<I/σ(I)>	6.6	1.4
Completeness [%]	100.0
Redundancy	14.2
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.08 M Sodium chloride; 0.1 M MES pH 6.5; 37 % v/v PEG 400; 4% ethylene glycol