6Z8C
Crystal Structure of the Voltage-Gated Sodium Channel NavMs (F208L) in complex with N-desmethyltamoxifen (3.2 A resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96864 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 109.221, 109.221, 211.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.510 - 3.200 |
R-factor | 0.2541 |
Rwork | 0.254 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hvx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.510 | 3.420 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 10937 | 1934 |
<I/σ(I)> | 6.6 | 1.4 |
Completeness [%] | 100.0 | |
Redundancy | 14.2 | |
CC(1/2) | 0.990 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.08 M Sodium chloride; 0.1 M MES pH 6.5; 37 % v/v PEG 400; 4% ethylene glycol |