6Z7X

Insulin analytical antibody OXI-005 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	84.190, 94.140, 52.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.920 - 2.150
R-factor	0.198
Rwork	0.195
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xkn
RMSD bond length	0.009
RMSD bond angle	1.018
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3714)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.920	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.088	1.127
Rmeas	0.096	1.224
Rpim	0.038	0.474
Number of reflections	23390	14881
<I/σ(I)>	12.75	1.54
Completeness [%]	99.9	99.91
Redundancy	6.4	6.6
CC(1/2)	0.999	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulfate, 20 % (w/v) PEG 3350