6Z7B
Variant Surface Glycoprotein VSGsur bound to suramin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.688, 79.219, 118.029 |
Unit cell angles | 90.00, 90.78, 90.00 |
Refinement procedure
Resolution | 48.350 - 1.860 |
R-factor | 0.1961 |
Rwork | 0.190 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6z79 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.350 | 1.926 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 81517 | 8077 |
<I/σ(I)> | 12.25 | 2.32 |
Completeness [%] | 99.9 | 99.77 |
Redundancy | 6.7 | 6.8 |
CC(1/2) | 0.997 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % (v/v) isopropanol |