6Z7A
Variant Surface Glycoprotein VSGsur
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 47.042, 71.062, 130.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.050 - 1.210 |
R-factor | 0.1669 |
Rwork | 0.166 |
R-free | 0.18660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6z79 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.541 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.050 | 1.250 |
High resolution limit [Å] | 1.210 | 1.210 |
Rmerge | 0.096 | |
Rmeas | 0.104 | |
Rpim | 0.041 | |
Number of reflections | 122573 | 7073 |
<I/σ(I)> | 8.57 | |
Completeness [%] | 91.4 | |
Redundancy | 6.1 | |
CC(1/2) | 0.998 | 0.174 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % (v/v) Isopropanol |