6Z70

Structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-10
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	72.100, 116.100, 137.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.986 - 2.000
R-factor	0.2659
Rwork	0.266
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fv6
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.760	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.086	1.113
Rmeas		1.217
Rpim		0.489
Number of reflections	78497	7755
<I/σ(I)>	9.4	1.8
Completeness [%]	99.8	99.8
Redundancy	5.8	6
CC(1/2)	0.999	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	295	0.1 M Sodium Citrate-Citric acid 30% PEG 500 DME (v/v)