6Z6V

Globular head of C1q in complex with the nanobody C1qNb75

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976200
Spacegroup name	P 2 21 21
Unit cell lengths	61.816, 119.744, 131.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.890 - 2.190
R-factor	0.1798
Rwork	0.178
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hzf
RMSD bond length	0.010
RMSD bond angle	1.393
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.900	2.270
High resolution limit [Å]	2.190	2.190
Number of reflections	48574	2516
<I/σ(I)>	15.7
Completeness [%]	94.7	50
Redundancy	12.7	6.1
CC(1/2)	0.999	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	0.1 M BIS-Tris pH 5.5, 25 % w/v PEG 3350