6Z6D

Crystal structure of the HK97 bacteriophage large terminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9198
Spacegroup name	C 1 2 1
Unit cell lengths	211.787, 39.146, 65.450
Unit cell angles	90.00, 103.39, 90.00

Refinement procedure

Resolution	103.230 - 2.200
R-factor	0.2072
Rwork	0.205
R-free	0.25420
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.258
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	XDS (Oct 15, 2015)
Phasing software	SHELXD (2013/2)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.230	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.136	0.842
Number of reflections	26796	2242
<I/σ(I)>	7.1	1.8
Completeness [%]	99.6	98.2
Redundancy	3.3
CC(1/2)	0.991	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.6 M ammonium sulfate, pH 7.6