6Z4X
Structure of the CAK complex form Chaetomium thermophilum bound to ATP-gamma-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.970, 84.903, 154.477 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 46.650 - 2.980 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6z3u |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.513 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.650 | 3.230 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.277 | 0.983 |
| Rpim | 0.179 | 0.634 |
| Number of reflections | 29053 | 1703 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 89.3 | 67.1 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.952 | 0.409 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 100 mM HEPES pH 7.0-8.0, 0.2 M sodium formate, 15-22% PEG 3350 |
