6Z3Y
CryoEM structure of horse sodium/proton exchanger NHE9 in an inward-facing conformation
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum LS |
Phase Plate | ZERNIKE PHASE PLATE |
Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.51 |
Software | RELION,EPU,CTFFIND |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |