6Z3U

Structure of the CAK complex form Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0723
Spacegroup name	P 1 21 1
Unit cell lengths	81.436, 85.240, 160.401
Unit cell angles	90.00, 96.93, 90.00

Refinement procedure

Resolution	75.870 - 2.600
R-factor	0.205
Rwork	0.203
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ua2
RMSD bond length	0.003
RMSD bond angle	0.507
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.900	2.816
High resolution limit [Å]	2.600	2.604
Rmerge	0.314	1.776
Rpim	0.198	1.164
Number of reflections	33808	1690
<I/σ(I)>	6.6	1.3
Completeness [%]	83.4	64.4
Redundancy	6.8	6.2
CC(1/2)	0.980	0.249

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	100 mM HEPES pH 7.0-8.0, 0.2 M sodium formate, 15-22% PEG 3350.