6Z32

Human cation-independent mannose 6-phosphate/IGF2 receptor domains 7-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2013-03-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.05811
Spacegroup name	P 41 21 2
Unit cell lengths	139.204, 139.204, 234.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.720 - 3.470
Rwork	0.261
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology models of D7 8 9 10 Crystal structure of D11 (1GP0)
RMSD bond length	0.007
RMSD bond angle	1.564
Data reduction software	xia2 (0.5.902)
Data scaling software	DIALS (1.14)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.720	3.590
High resolution limit [Å]	3.470	3.470
Number of reflections	30224	2944
<I/σ(I)>	6.03	0.84
Completeness [%]	98.5	98.43
Redundancy	14.5	14.9
CC(1/2)	0.997	0.127

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M MES pH 6.5, 1.6 M MgSO4, 10 mM mannose 6-phosphate