6Z2G

Crystal structure of human NLRP9 PYD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000089
Spacegroup name	P 65 2 2
Unit cell lengths	33.330, 33.330, 311.091
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.860 - 1.950
R-factor	0.216
Rwork	0.215
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ewi
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.020
High resolution limit [Å]	1.950	1.950
Rmeas	0.082	1.000
Rpim	0.018	0.240
Number of reflections	14097	2285
<I/σ(I)>	18.5	1.8
Completeness [%]	99.5	99.2
Redundancy	22	16
CC(1/2)	0.997	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	288	0.1 M Bicine pH 9.0, 0.2 M sodium thiocyanate, 0.1 M sodium formate, and 25-27% v/w PEG3350