6Z2F
Crystal structure of human AGX1 mutant complexed with UDPGLCNAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976280 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.614, 64.569, 82.070 |
| Unit cell angles | 95.63, 101.09, 104.93 |
Refinement procedure
| Resolution | 29.260 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jv1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.560 | 29.260 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.300 | 1.700 |
| Rmerge | 0.043 | 0.024 | 0.429 |
| Rmeas | 0.051 | 0.029 | 0.518 |
| Rpim | 0.027 | 0.015 | 0.286 |
| Total number of observations | 1833 | 13668 | |
| Number of reflections | 93417 | 560 | 4323 |
| <I/σ(I)> | 16.4 | 46.3 | 2.6 |
| Completeness [%] | 96.5 | 95.4 | 90.9 |
| Redundancy | 3.5 | 3.3 | 3.2 |
| CC(1/2) | 0.999 | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M MgCl2, 0.1 M Tris, pH 8.5, 30% PEG 4000 |
