6Z2D
Crystal structure of wild type OgpA from Akkermansia muciniphila in P 41 21 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.968820 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.861, 64.861, 187.426 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.024 - 1.899 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.22100 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.024 | 1.967 |
High resolution limit [Å] | 1.899 | 1.899 |
Rmerge | 0.092 | 0.840 |
Rmeas | 0.095 | 0.840 |
Number of reflections | 32350 | 2963 |
<I/σ(I)> | 26.95 | |
Completeness [%] | 99.3 | |
Redundancy | 24.9 | 21.1 |
CC(1/2) | 1.000 | 0.687 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM sodium HEPES/MOPS pH 7.5, 100 mM carboxylic acids mixture (sodium formate, ammonium acetate, sodium citrate tribasic dihydrate, sodium potassium tartrate tetrahydrate and sodium oxamate), 20% PEG 500 MME and 10% PEG 20000 |