6YXZ
Structure and activity of the GH20 beta-N-beta-N-acetylhexosaminidase from Bifidobacterium bifidum
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.566, 126.750, 152.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.380 - 1.750 |
| Rwork | 0.160 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | generated by MrBUMP |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.511 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.380 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 1.654 | |
| Rpim | 0.969 | |
| Number of reflections | 111519 | 5474 |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.4 |
| CC(1/2) | 0.997 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 21.6 mg/mL of purified protein in the buffer of tris 25 mM pH 8.0 and NaCl 200 mM is mixed with PEG 3350 23%, 0.1 M bis-tris propane PH 6.5 and 0.2 M NaBr at 1:1 ratio. |
