6YXF

Cryogenic human adiponectin receptor 2 (ADIPOR2) with Gd-DO3 ligand determined by Serial Crystallography (SSX) using CrystalDirect

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.72
Spacegroup name	P 21 2 21
Unit cell lengths	74.640, 100.650, 111.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.670 - 3.025
R-factor	0.2163
Rwork	0.214
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lwy
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (29-NOV-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.000	3.010
Number of reflections	31468	2336
<I/σ(I)>	5.06
Completeness [%]	100.0
Redundancy	14.98
CC(1/2)	0.980	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	30 or 50 nl bolus overlaid with 600 nl precipitant solution using either a Mosquito LCP robot (SPT Labtech) in CrystalDirect plate-2. The crystallisation condition is made of 42.3% PEG 400, 110 mM potassium citrate, 100 mM HEPES pH 7.0. 60-120 nL of Gd-DO3 soaking solution with a final conc. of 10 - 25 mM was delivered onto LCP crystallisation drop. Crystallisation experiments were carried out at the HTX facility of EMBL Grenoble. LCP bolus were harvested automatically at cryogenic condition using CrystalDirect technology.