6YVH
CWC22-CWC27-EIF4A3 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 151.255, 163.627, 181.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.690 - 3.190 |
| R-factor | 0.2334 |
| Rwork | 0.232 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c9b |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.710 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3758) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.690 | 3.360 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.122 | |
| Number of reflections | 143366 | 97793 |
| <I/σ(I)> | 14.6 | 1.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.3 | |
| CC(1/2) | 0.999 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292.15 | 20% (w/v) PEG20000, 50 mM MES pH 6.5 |
