6YVE
Glycogen phosphorylase b in complex with pelargonidin 3-O-beta-D-glucoside
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 126.147, 126.147, 115.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 126.150 - 2.100 |
Rwork | 0.169 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.548 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.150 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.085 | 0.530 |
Number of reflections | 54889 | 42833 |
<I/σ(I)> | 14.7 | |
Completeness [%] | 99.9 | |
Redundancy | 9.3 | |
CC(1/2) | 0.998 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.8 | 289 | 10mM BES buffer pH 6.7 |