6YTU

Atomic-resolution structure of the coiled-coil dimerisation domain of human Arc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	26.844, 42.556, 67.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 0.950
R-factor	0.1042
Rwork	0.103
R-free	0.12410
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.708
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	0.970
High resolution limit [Å]	0.950	0.950
Rmeas	0.085	1.455
Number of reflections	44960	1366
<I/σ(I)>	18.7	1
Completeness [%]	90.7	38
Redundancy	10.8	4.3
CC(1/2)	0.907	0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	281	45% MPD, 0.1 M Tris-HCl (pH 7.5)