6YTB

GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	49.158, 75.218, 149.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.700 - 1.650
R-factor	0.2176
Rwork	0.216
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hy9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3893)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.850	1.730
High resolution limit [Å]	1.650	1.650
Rpim	0.024	0.568
Number of reflections	61292	3069
<I/σ(I)>	16.2	1.3
Completeness [%]	95.1	51.5
Redundancy	6.6	6.6
CC(1/2)	1.000	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	20% (w/v) PEG3350, 0.2M potassium formate