6YSY

Skeletal Myosin bound to MPH-220, MgADP-VO4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980102
Spacegroup name	P 21 21 21
Unit cell lengths	49.607, 119.424, 176.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.930 - 3.246
R-factor	0.2183
Rwork	0.217
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qvi
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.930	3.400
High resolution limit [Å]	3.246	3.246
Rmerge	0.004	0.229
Number of reflections	17314	1687
<I/σ(I)>	10.46
Completeness [%]	99.8	99.88
Redundancy	2
CC(1/2)	0.999	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	35% PEG600, 20mM DTT; 100mM HEPES pH 7.0; 5% DMSO